GreenWater Laboratories is one of the first commercial laboratories with the expertise to perform and interpret cyanotoxin analyses. All analyses are conducted by professionals in the field of chemistry with rigorous QA/QC. Confirmatory analyses are available upon request (e.g. LC/MS/(MS) for ELISA assays).
(Enzyme linked immunosorbent assay)
ELISAs are very popular screening assays that provide limited quantitative information for specific groups of cyanotoxins. ELISAs typically have the ability to detect toxins at low levels for both surface and drinking waters. It should be noted that ELISAs are also prone to sample matrix interference that may confound results, leading to erroneous conclusions. It is important to have the ability to verify any positive ELISA responses with other techniques, such as HPLC coupled with PDA, FL or MS.
| Algal Toxin | Reporting Limit ng/mL (ppb) |
Method |
|---|---|---|
| Microcystins/Nodularins | 0.15 | Adda ELISA (Method 546 & Ohio EPA DES 701.0) |
| Cylindrospermopsin | 0.10 | Cylindrospermopsin (522011) |
| Saxitoxin | 0.05 | STX specific assay (52255B) |
| Brevetoxin (NSP) | 0.10 | PbTx3 specific assay (520026) |
| Okadaic Acid (DSP) | 0.05 | OA specific assay (520021) |
| Anatoxin-a(s) (OP carbamate) | qualitative screen | Organophosphate / Carbamate specific assay(550051) |
(High Performance Liquid Chromatography
GWL/CL currently maintains and operates multiple high performance liquid chromatography (HPLC) systems with photodiode array (PDA), fluorescence (FL), and mass spectrometry (Ion Trap and Triple Quad) detection. This provides GWL/CL the ability to report data with a high level of confidence as well as the ability to supply confirmatory analysis on ELISA data.
Algal Toxin |
Method Reporting Limits (ppb) |
Method |
|---|---|---|
Microcystins/Nodularin[DAsp3]MC-RR, MC-RR, NOD, MC-YR, MC-HtYR, MC-LR, [DAsp3]MC-LR, [Dha7]MC-LR, MC-HilR, MC-WR, [DLeu1]MC-LR, MC-LY, MC-LA, MC-LW, MC-LF |
0.01-0.10 | Modification of Foss & Aubel (2015) to include more variants |
Total Microcystins using MMPB |
0.05 | Per Foss & Aubel (2015) |
Cylindrospermopsin / Epi-cylindrospermopsin |
0.05 | Modification of Foss & Aubel (2013) |
Anatoxin-a / Homoanatoxin-a |
0.05 | Modification of Method 545 to include homoanatoxin-a |
Anatoxin-a / Cylindrospermopsin |
0.03 / 0.09 | Method 545 |
Saxitoxins (Paralytic Shellfish Toxins)Including analogs: STX, dcSTX, NEO, C1C2, GTX 1,2,3,4,5, dcGTX23 |
0.02-0.6 | Modification of SeQuant Technical Note TN-009 using ZIC-HILIC |
BMAA (includes isomers BAMA, DAB & AEG) |
0.5-10.0 | In-house method developed using ZIC-HILIC chromatography |
Domoic Acid |
0.05 | In house method developed using Triple-Quadropole MS/MS |
Lyngbyatoxin-A with Aplysiatoxin/Debromoaplysiatoxin |
0.10 (LA), 1 (AT), 5 (DAT) | In house method developed using Triple-Quadropole MS/MS |
Method Reporting Limits are based on water samples and may vary depending on spike (LFM) returns. Other toxin analyses are available, such as the AOAC 2005.06 fluorescence method for PSTs. Please call regarding your specific needs.
Rapid turnaround times are available on most toxin analyses conducted on water samples. Please call for rapid TAT requests.
GreenWater Laboratories conducts cyanotoxin analyses on many different biological matrices, including tissues and food products. We have developed many methods to address exposure to HABs and you can find our research on our articles page. For more information on analyzing biological matrices, click here.
Additonal extraction charges do apply to complicated matrices. Please contact us with specific requests.
Please fill out the linked Analysis Request Form and ship it with your samples. This will also serve as a chain of custody (COC).
The linked document explains some of our services, including descriptions of the different types of analyses we conduct.
For prompt quotes and timeframes for our services, please call or e-mail us at info@greenwaterlab.com.